Ecosyste.ms: Papers
An open API service providing mapping between scientific papers and software projects that are mentioned in them.
All mentions data is based on the CZI Software Mentions dataset.
Projects: pypi: real
https://packages.ecosyste.ms/registries/pypi.org/packages/real
1 version
Latest release: over 1 year ago
Papers Mentioning real 66
10.7554/eLife.60265
The cryo-EM structure of the human uromodulin filament core reveals a unique assembly mechanismCited by: 20
Author(s): Jessica J. Stanisich, Dawid Zyla, Pavel Afanasyev, Jianlin Xu, Anne Kipp, Eric Olinger, Olivier Devuyst, Martin Pilhofer, Daniel Boehringer, Rudi Glockshuber
Software Mentions: 6
Published: over 4 years ago
10.1107/S1399004714028132
FEM: feature-enhanced mapCited by: 155
Author(s): Pavel V. Afonine, Nigel W. Moriarty, Marat Mustyakimov, Oleg Sobolev, Thomas C. Terwilliger, Dušan Turk, Alexandre Urzhumtsev, Paul D. Adams
Software Mentions: 5
Published: almost 10 years ago
10.1107/S2059798318006551
Real-space refinement in<i>PHENIX</i>for cryo-EM and crystallographyCited by: 1,815
Author(s): Pavel V. Afonine, Billy K. Poon, Randy J. Read, Oleg Sobolev, Thomas C. Terwilliger, Alexandre Urzhumtsev, Paul D. Adams
Software Mentions: 5
Published: over 6 years ago
10.1371/journal.pone.0240932
Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1Cited by: 27
Author(s): Guillaume Gaullier, Genevieve Roberts, Uma M. Muthurajan, Samuel Bowerman, Johannes Rudolph, Jyothi Mahadevan, Asmita Jha, Purushka S. Rae, Karolin Luger
Software Mentions: 5
Published: about 4 years ago
10.1107/S2059798318002425
<i>ISOLDE</i>: a physically realistic environment for model building into low-resolution electron-density mapsCited by: 933
Author(s): Tristan I. Croll
Software Mentions: 5
Published: over 6 years ago
10.18632/oncotarget.25218
Comprehensive identification of long noncoding RNAs in colorectal cancerCited by: 15
Author(s): Eric James de Bony, Martin Bizet, Olivier Van Grembergen, Bouchra Hassabi, Emilie Calonne, Pascale Putmans, Gianluca Bontempi, François Fuks
Software Mentions: 5
Published: over 6 years ago
10.7554/eLife.46070
Large protein organelles form a new iron sequestration system with high storage capacityCited by: 83
Author(s): Tobias W. Giessen, Benjamin J. Orlando, Andrew A Verdegaal, Melissa G. Chambers, Jules Gardener, David C. Bell, Gabriel Birrane, Maofu Liao, Pamela A. Silver
Software Mentions: 5
Published: over 5 years ago
10.1038/s41598-018-31850-3
Structures of Mycobacterium smegmatis 70S ribosomes in complex with HPF, tmRNA, and P-tRNACited by: 31
Author(s): Satabdi Mishra, Tofayel Ahmed, Anu Tyagi, Jing Shi, Shashi Bhushan
Software Mentions: 4
Published: over 6 years ago
10.1126/sciadv.abg9923
Structure of the mycobacterial ESX-5 type VII secretion system pore complexCited by: 38
Author(s): Katherine S. H. Beckham, Christina Ritter, Grzegorz Chojnowski, Daniel S. Ziemianowicz, Edukondalu Mullapudi, Mandy Rettel, Mikhail M. Savitski, Simon A. Mortensen, Jan Kosiński, Matthias Wilmanns
Software Mentions: 4
Published: over 3 years ago
10.1107/S2059798320015879
<i>CERES</i>: a cryo-EM re-refinement system for continuous improvement of deposited modelsCited by: 11
Author(s): Dorothée Liebschner, Pavel V. Afonine, Nigel W. Moriarty, Billy K. Poon, Vincent B. Chen, Paul D. Adams
Software Mentions: 4
Published: almost 4 years ago
10.7554/eLife.38888
Structural principles of SNARE complex recognition by the AAA+ protein NSFCited by: 68
Author(s): K. Ian White, Minglei Zhao, Ucheor B. Choi, Richard A. Pfuetzner, Axel T. Brunger
Software Mentions: 4
Published: over 6 years ago
10.7554/eLife.32481
Molecular structure of human KATP in complex with ATP and ADPCited by: 127
Author(s): Kenneth Pak Kin Lee, Jue Chen, Roderick MacKinnon
Software Mentions: 4
Published: almost 7 years ago
10.1126/sciadv.aax8286
Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunityCited by: 53
Author(s): Ni Tien, Fang Jiao, Xiulian Yu, Saša Aden, Lucy Ginger, Sophie I. Williams, Fangfang Bai, Vojtěch Pražák, Dimple Karia, Phillip J. Stansfeld, Peijun Zhang, George P. Munson, Gregor Anderluh, Simon Scheuring, R.J.C. Gilbert
Software Mentions: 4
Published: almost 5 years ago
10.1107/S2059798318009324
New tools for the analysis and validation of cryo-EM maps and atomic modelsCited by: 536
Author(s): Pavel V. Afonine, Bruno P. Klaholz, Nigel W. Moriarty, Billy K. Poon, Oleg V. Sobolev, Thomas C. Terwilliger, Paul D. Adams, Alexandre Urzhumtsev
Software Mentions: 4
Published: over 6 years ago
10.1107/S2052252519007619
<i>Namdinator</i> – automatic molecular dynamics flexible fitting of structural models into cryo-EM and crystallography experimental mapsCited by: 242
Author(s): Rune T. Kidmose, Jonathan Juhl, Poul Nissen, Thomas Boesen, Jesper Lykkegaard Karlsen, Bjørn Panyella Pedersen
Software Mentions: 3
Published: over 5 years ago
10.3390/s16071132
On Inertial Body Tracking in the Presence of Model Calibration ErrorsCited by: 75
Author(s): Markus Miezal, Bertram Taetz, Gabriele Bleser
Software Mentions: 3
Published: over 8 years ago
10.7554/eLife.52983
The structure of the endogenous ESX-3 secretion systemCited by: 53
Author(s): Nicole Poweleit, Nadine Czudnochowski, Rachel Nakagawa, Donovan Trinidad, Kenan C. Murphy, Christopher M. Sassetti, Oren S. Rosenberg
Software Mentions: 3
Published: almost 5 years ago
10.1016/j.molcel.2020.04.012
Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication ForkCited by: 100
Author(s): Domagoj Baretić, Michael Jenkyn-Bedford, Valentina Aria, Giuseppe Cannone, Mark Skehel, Joseph T.P. Yeeles
Software Mentions: 3
Published: over 4 years ago
10.7554/eLife.50175
Ligand recognition and gating mechanism through three ligand-binding sites of human TRPM2 channelCited by: 64
Author(s): Yongyang Huang, Becca Roth, Wei Lü, Juan Du
Software Mentions: 3
Published: over 5 years ago
10.7554/eLife.46417
Mechanism of pharmacochaperoning in a mammalian KATP channel revealed by cryo-EMCited by: 58
Author(s): Gregory M. Martin, Min Woo Sung, Zhongying Yang, Laura Innes, Krishnaswamy Balamurugan, Larry L. David, Craig Yoshioka, Show Ling Shyng
Software Mentions: 3
Published: over 5 years ago
10.1371/journal.pbio.3000958
Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosomeCited by: 14
Author(s): Kai Ehrenbolger, Nathan Jespersen, Himanshu Sharma, Yuliya Y. Sokolova, Yuri S. Tokarev, Charles R. Vossbrinck, Jonas Barandun
Software Mentions: 3
Published: about 4 years ago
10.7554/eLife.53243
Cryo-EM structures demonstrate human IMPDH2 filament assembly tunes allosteric regulationCited by: 42
Author(s): Matthew C. Johnson, Justin M. Kollman
Software Mentions: 3
Published: almost 5 years ago
10.7554/eLife.22086
Molecular architecture of the 90S small subunit pre-ribosomeCited by: 116
Author(s): Qiaoyan Sun, Xing Zhu, Qiong Jia, Wenling An, Pengfei Lan, Dan Tan, Rongchang Chen, Bing Wang, Sanduo Zheng, Cheng Zhang, Xining Chen, Wei Zhang, Jing Chen, Meng‐Qiu Dong, Keqiong Ye
Software Mentions: 3
Published: almost 8 years ago
10.1038/cr.2017.124
A high-quality genome assembly of quinoa provides insights into the molecular basis of salt bladder-based salinity tolerance and the exceptional nutritional valueCited by: 152
Author(s): Changsong Zou, A Chen, Li Xiao, H.-A. Müller, Peter Ache, Georg Haberer, M Zhang, Jia Wei, Ping Deng, Ru Huang, Daniel Lang, Li Feng, Dong Zhan, Xiaoyong Wu, H Zhang, Jennifer Böhm, Ruirui Liu, Lana Shabala, Rainer Hedrich, J-K Zhu
Software Mentions: 3
Published: about 7 years ago
10.1038/s42003-021-01919-3
High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beamsCited by: 39
Author(s): Koji Kato, Naoyuki Miyazaki, Tasuku Hamaguchi, Yoshiki Nakajima, Fusamichi Akita, Koji Yonekura, Jian‐Ren Shen
Software Mentions: 3
Published: almost 4 years ago
10.15252/embj.2018100825
Diverse roles of TssA‐like proteins in the assembly of bacterial type VI secretion systemsCited by: 34
Author(s): Johannes Schneider, Sergey Nazarov, Ricardo Adaixo, Martina Liuzzo, Peter David Ringel, Henning Stahlberg, Marek Basler
Software Mentions: 3
Published: over 5 years ago
10.7554/eLife.59018
Structural ordering of the Plasmodium berghei circumsporozoite protein repeats by inhibitory antibody 3D11Cited by: 10
Author(s): Iga Kucharska, Elaine Thai, Ananya Srivastava, John L. Rubinstein, Régis Pomès, Jean-Philippe Julien
Software Mentions: 3
Published: about 4 years ago
10.1038/s41421-021-00259-0
Structure of the full-length human Pannexin1 channel and insights into its role in pyroptosisCited by: 11
Author(s): Sensen Zhang, Baolei Yuan, Jordy Homing Lam, Jun Zhou, Xuan Zhou, Gerardo Ramos‐Mandujano, Xin‐Xia Tian, Yang Liu, Han Ren, Li Yu, Xin Gao, Maojun Yang
Software Mentions: 2
Published: over 3 years ago
10.3390/molecules26020399
Identification of Phytochemical-Based β-Catenin Nuclear Localization Inhibitor in NSCLC: Differential Targeting Population from Member of IsothiocyanatesCited by: 9
Author(s): Win Sen Heng, Shiau‐Chuen Cheah
Software Mentions: 2
Published: almost 4 years ago
10.1371/journal.pone.0136820
Reference Gene Selection for qPCR Analysis in Tomato-Bipartite Begomovirus Interaction and Validation in Additional Tomato-Virus PathosystemsCited by: 40
Author(s): A. L. M. Lacerda, Leonardo N. Fonseca, Rosana Blawid, L. S. Boiteux, S. G. Ribeiro, Ana Cristina Miranda Brasileiro
Software Mentions: 2
Published: over 9 years ago
10.15252/embr.201948451
Elucidation of the viral disassembly switch of tobacco mosaic virusCited by: 18
Author(s): Félix Weis, Maximilian Beckers, Iris von der Hocht, Carsten Sachse
Software Mentions: 2
Published: over 5 years ago
10.1126/sciadv.aba8381
Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesiclesCited by: 68
Author(s): Oleksiy Kovtun, Veronica Kane Dickson, Bernard T. Kelly, David J. Owen, John Briggs
Software Mentions: 2
Published: over 4 years ago
10.7554/eLife.46041
Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the gβ subunitCited by: 50
Author(s): Ching‐Ju Tsai, Jacopo Marino, Ricardo Adaixo, Filip Pamula, J. Muehle, Shoji Maeda, Tilman Flock, Nicholas M. I. Taylor, I. Mohammed, Hugues Matile, Roger Dawson, Xavier Deupí, Henning Stahlberg, Gebhard F. X. Schertler
Software Mentions: 2
Published: over 5 years ago
10.7554/eLife.45185
Conformational switches control early maturation of the eukaryotic small ribosomal subunitCited by: 32
Author(s): Mirjam Hunziker, Jonas Barandun, Olga Buzovetsky, Caitlin Steckler, Henrik Molina, Sebastian Klinge
Software Mentions: 2
Published: over 5 years ago
10.7554/eLife.28384
Cryo-EM structure of the SAGA and NuA4 coactivator subunit Tra1 at 3.7 angstrom resolutionCited by: 35
Author(s): Luis Miguel Díaz-Santín, Natalya Lukoyanova, Emir Aciyan, Alan C. M. Cheung
Software Mentions: 2
Published: over 7 years ago
10.7554/eLife.56862
Mechanical inhibition of isolated Vo from V/A-ATPase for proton conductanceCited by: 11
Author(s): Jun-ichi Kishikawa, Atsuko Nakanishi, Aya Furuta, Takayuki Kato, Keiichi Namba, Masatada Tamakoshi, Kaoru Mitsuoka, Ken Yokoyama
Software Mentions: 2
Published: over 4 years ago
10.7554/eLife.39775
Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structuresCited by: 36
Author(s): Jan Rheinberger, Xiaolong Gao, Philipp A. M. Schmidpeter, Crina M. Nimigean
Software Mentions: 2
Published: over 6 years ago
10.7554/eLife.55954
Near-atomic structures of the BBSome reveal the basis for BBSome activation and binding to GPCR cargoesCited by: 32
Author(s): Shuang Yang, Kriti Bahl, Hui‐Ting Chou, Jonathan Woodsmith, Ulrich Stelzl, Thomas Walz, Maxence V. Nachury
Software Mentions: 2
Published: over 4 years ago
10.7554/eLife.68806
Visualizing formation of the active site in the mitochondrial ribosomeCited by: 19
Author(s): Viswanathan Chandrasekaran, Nirupa Desai, Nick Burton, Hongxin Yang, Jonathan Price, Eric A. Miska, V. Ramakrishnan
Software Mentions: 2
Published: about 3 years ago
10.7554/eLife.71188
Analysis of the mechanosensor channel functionality of TACANCited by: 19
Author(s): Yiming Niu, Xiao Tao, George Vaisey, Paul Dominic B. Olinares, Hanan Alwaseem, Brian T. Chait, Roderick MacKinnon
Software Mentions: 2
Published: over 3 years ago
10.7554/eLife.70871
Regulation of human mTOR complexes by DEPTORCited by: 11
Author(s): Matthias Wälchli, Karolin Berneiser, Francesca Mangia, Stefan Imseng, Louise-Marie Craigie, Edward Stuttfeld, Michael N. Hall, Timm Maier
Software Mentions: 2
Published: over 3 years ago
10.7554/eLife.60482
Structure of the bacterial ribosome at 2 Å resolutionCited by: 125
Author(s): Zoe L Watson, Fred R. Ward, Raphaël Méheust, Omer Ad, Alanna Schepartz, Jillian F. Banfield, Jamie H. D. Cate
Software Mentions: 2
Published: over 4 years ago
10.7554/eLife.62611
Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clientsCited by: 57
Author(s): Lakshmi E. Miller-Vedam, Bastian Bräuning, Katerina D. Popova, Nicole T. Schirle Oakdale, Jessica L. Bonnar, J.R. Prabu, Elizabeth A. Boydston, Natalia Sevillano, Matthew J. Shurtleff, Robert M. Stroud, Charles S. Craik, Brenda A. Schulman, Adam Frost, Jonathan S. Weissman
Software Mentions: 2
Published: about 4 years ago
10.1371/journal.ppat.1006607
Structures of foot and mouth disease virus pentamers: Insight into capsid dissociation and unexpected pentamer reassociationCited by: 21
Author(s): Nayab Malik, Abhay Kotecha, Sarah Gold, Amin S. Asfor, Jingshan Ren, Juha T. Huiskonen, Tobias J. Tuthill, Elizabeth E. Fry, David I. Stuart
Software Mentions: 1
Published: over 7 years ago
10.7554/eLife.25642
The force-sensing peptide VemP employs extreme compaction and secondary structure formation to induce ribosomal stallingCited by: 74
Author(s): Ting Su, Jingdong Cheng, Daniel Sohmen, Rickard Hedman, Otto Berninghausen, Gunnar von Heijne, Daniel N. Wilson, Roland Beckmann
Software Mentions: 1
Published: over 7 years ago
10.7554/eLife.65703
eIF2B conformation and assembly state regulate the integrated stress responseCited by: 39
Author(s): Michael Schoof, Morgane Boone, Lan Wang, Rosalie Lawrence, Adam Frost, Peter Walter
Software Mentions: 1
Published: almost 4 years ago
10.3390/ani9121076
Transcriptome of Chicken Liver Tissues Reveals the Candidate Genes and Pathways Responsible for Adaptation into Two Different Climatic ConditionsCited by: 9
Author(s): Himansu Kumar, Asankadyr U. Iskender, Krishnamoorthy Srikanth, Hana Kim, Asankadyr Zhunushov, Hyojun Chooq, Gul Won Jang, Youngjo Lim, Ki Duk Song
Software Mentions: 1
Published: about 5 years ago
10.1038/s42003-020-01217-4
Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome releaseCited by: 7
Author(s): Hisashi Naitow, Tasuku Hamaguchi, Saori Maki-Yonekura, Masamichi Isogai, Nobuyuki Yoshikawa, Koji Yonekura
Software Mentions: 1
Published: over 4 years ago
10.1038/s42003-021-02222-x
3D architecture and structural flexibility revealed in the subfamily of large glutamate dehydrogenases by a mycobacterial enzymeCited by: 3
Author(s): Melisa Lázaro, Roberto Melero, Charlotte Huet, Jorge P. López‐Alonso, Sandra Delgado, Alexandra Dodu, Eduardo M. Bruch, Luciano A. Abriata, P.M. Alzari, Mikel Valle, María‐Natalia Lisa
Software Mentions: 1
Published: over 3 years ago
10.1371/journal.pone.0210749
Structure basis of neutralization by a novel site II/IV antibody against respiratory syncytial virus fusion proteinCited by: 5
Author(s): Qingqing Xie, Zhao Wang, Fengyun Ni, Hong Chen, Jianpeng Ma, Nita Patel, Huanzhang Lü, Ye Liu, Jing-Hui Tian, David C. Flyer, Michael J. Massare, Larry Ellingsworth, Gregory M. Glenn, Gale Smith, Qinghua Wang
Software Mentions: 1
Published: almost 6 years ago
10.7554/eLife.28560
Structure of RNA polymerase bound to ribosomal 30S subunitCited by: 79
Author(s): Gabriel Demo, Aviram Rasouly, Nikita Vasilyev, Vladimir Svetlov, A.B. Loveland, Rubén Díaz-Avalos, Nikolaus Grigorieff, А.A. Коростелев
Software Mentions: 1
Published: about 7 years ago
10.1186/1471-2164-12-313
Chromosome-wide mapping of DNA methylation patterns in normal and malignant prostate cells reveals pervasive methylation of gene-associated and conserved intergenic sequencesCited by: 65
Author(s): Srinivasan Yegnasubramanian, Zhijin Wu, Michael C. Haffner, David Esopi, Martin J. Aryee, Raghav Badrinath, Tony L. He, James D. Morgan, Benilton Carvalho, Qizhi Zheng, Angelo M. De Marzo, Rafael A. Irizarry, William G. Nelson
Software Mentions: 1
Published: over 13 years ago
10.1371/journal.pone.0181240
Emergent multisystemic Enterococcus infection threatens endangered Christmas Island reptile populationsCited by: 15
Author(s): Karrie Rose, Jessica E Agius, Jane Hall, Peggy Thompson, John‐Sebastian Eden, Mukesh Srivastava, Brendan Tiernan, Cheryl Jenkins, David N. Phalen
Software Mentions: 1
Published: over 7 years ago
10.7554/eLife.54575
A complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediateCited by: 13
Author(s): Ritam Neupane, Vera P. Pisareva, Carlos Fernández Rodríguez, Andrey V. Pisarev, I.S. Fernandez
Software Mentions: 1
Published: over 4 years ago
10.1128/mBio.00818-19
The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion SystemsCited by: 48
Author(s): Steven Johnson, Lucas Kuhlen, Justin C. Deme, Patrizia Abrusci, Susan M. Lea
Software Mentions: 1
Published: over 5 years ago
10.1107/S2052252520013482
High-resolution cryo-EM using beam-image shift at 200 keVCited by: 13
Author(s): Jennifer N. Cash, Sarah Kearns, Yilai Li, Michael A. Cianfrocco
Software Mentions: 1
Published: about 4 years ago
10.1017/qrd.2020.16
A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particlesCited by: 9
Author(s): Kaiming Zhang, Shanshan Li, Grigore Pintilie, David Chmielewski, Michael F. Schmid, Graham Simmons, Jing Jin, Wah Chiu
Software Mentions: 1
Published: almost 5 years ago
10.7717/peerj.7769
Multiplexed ISSR genotyping by sequencing distinguishes two precious coral species (Anthozoa: Octocorallia: Coralliidae) that share a mitochondrial haplotypeCited by: 19
Author(s): Kenji Takata, Hiroki Taninaka, Manabu Nonaka, Fumihito Iwase, Taisei Kikuchi, Yoshihisa Suyama, Satoshi Nagai, Nina Yasuda
Software Mentions: 1
Published: about 5 years ago
10.7554/eLife.38686
Distinct and evolutionary conserved structural features of the human nuclear exosome complexCited by: 40
Author(s): Piotr Gerlach, Jan M. Schuller, Fabien Bonneau, J. Basquin, Peter Reichelt, Sebastian Falk, Elena Conti
Software Mentions: 1
Published: over 6 years ago
10.7554/eLife.50003
A structural mechanism for phosphorylation-dependent inactivation of the AP2 complexCited by: 14
Author(s): Edward A Partlow, Richard W. Baker, Gwendolyn M Beacham, Joshua S. Chappie, Andres E. Leschziner, Gunther Hollopeter
Software Mentions: 1
Published: over 5 years ago
10.3390/v12090953
Structure of the Capsid Size-Determining Scaffold of “Satellite” Bacteriophage P4Cited by: 12
Author(s): James L. Kizziah, Cynthia M. Rodenburg, Terje Dokland
Software Mentions: 1
Published: over 4 years ago
10.1007/s13238-017-0476-5
Cryo-EM structures of the mammalian endo-lysosomal TRPML1 channel elucidate the combined regulation mechanismCited by: 35
Author(s): Sensen Zhang, Ningning Li, Wenwen Zeng, Ning Gao, Maojun Yang
Software Mentions: 1
Published: over 7 years ago
10.1371/journal.ppat.1008263
Cryo-EM structure of the Shigella type III needle complexCited by: 32
Author(s): Michele Lunelli, Antje Kamprad, Jörg Bürger, Thorsten Mielke, C.M.T. Spahn, Michael Kolbe
Software Mentions: 1
Published: almost 5 years ago
10.7554/eLife.33660
Structure-based membrane dome mechanism for Piezo mechanosensitivityCited by: 275
Author(s): Yusong R. Guo, Roderick MacKinnon
Software Mentions: 1
Published: about 7 years ago
10.7554/eLife.23687
Mechanism of ribosome rescue by ArfA and RF2Cited by: 47
Author(s): Gabriel Demo, Egor Svidritskiy, Rohini Madireddy, Rubén Díaz-Avalos, Tim Grant, Nikolaus Grigorieff, Duncan Sousa, А.A. Коростелев
Software Mentions: 1
Published: almost 8 years ago